. 2017 Jun 1;12(6):e0178355. doi: 10.1371/journal.pone.0178355

Table 2. BAL fluid cell counts (x10³) (mean ±SEM) in mice at day 1, 3, 28 and 90 post exposure to VC (0.1% TW80), GO, rGO or Printex90 at doses 0, 18, 54 or 162 μg/mouse (n = 7–8).

	Dose	Neutrophils^a	Macrophages^a	Eosinophils	Lymphocytes	Total^a
Day 1
VC	0	8.5 ± 2.6	63 ± 19.4	0.2 ± 0.1	1 ± 0.8	72.6 ± 20.7
GO	18	219.5 ± 18.7^***^#	59.8 ± 5.6	5.7 ± 3.9	1.4 ± 0.9	294.7 ± 20.7^***
	54	94.9 ± 6.4^***^#	29.1 ± 4.1^*	0.5 ± 0	0 ± 0	123.9 ± 8.8^***
	162	120.7 ± 60.6^***	92.4 ± 8.5	96.2 ± 93.6	0 ± 0	309.3 ± 109.7^***
rGO	18	5.1 ± 1.4	44.4 ± 9.2	0.5 ± 0.3	0.5 ± 0.2	20.2 ± 10.5
	54	10 ± 1.8	61.7 ± 7	1.4 ± 0.2	0.4 ± 0.2	73.5 ± 7.8
	162	43.3 ± 7.5^***	68.7 ± 9.6	5.3 ± 2.5	0.1 ± 0.1	117.4 ± 16.1
P90	162	105.6 ± 19.5^***	24.4 ± 9.4	5.5 ± 2.3	0 ± 0	135.6 ± 17.1^*
Day 3
VC	0	0.5 ± 0.2	32.9 ± 3.9	0 ± 0	0 ± 0	33.5 ± 3.9
GO	18	38.8 ± 8.6^***^#	78.7 ± 6^***	51.3 ± 14.6	2.9 ± 1	171.7 ± 22.4^***
	54	324.7 ± 100.8^***^#	171.2 ± 36^***	10.8 ± 9.7	8.7 ± 2.2	515.5 ± 141.6^***
	162	574.4 ± 142.9^***^#	149.6 ± 15.9^***	0.6 ± 0.6	2.2 ± 1.4	727 ± 158.8^***
rGO	18	5.7 ± 4.9	43.2 ± 5.1	12.5 ± 12.2	0.9 ± 0.8	62.2 ± 21.9
	54	3 ± 1	49.4 ± 7.1	1.6 ± 0.4	0.3 ± 0.2	54.2 ± 7.9
	162	5.3 ± 1.6^*	50.1 ± 7.1	11.6 ± 5.8	0.2 ± 0.1	67.1 ± 11.5^*
P90	162	101.3 ± 22.3^***	55.7 ± 7.2^*	5.5 ± 2	0.8 ± 0.6	163.3 ± 26.4^***
Day 28
VC	0	0.9 ± 0.3	17.7 ± 5.4	0 ± 0	0.1 ± 0.1	18.7 ± 5.5
GO	18	0.7 ± 0.2	45 ± 2.8^**	0 ± 0	1 ± 0.5	46.7 ± 2.9^**
rGO	18	2.1 ± 0.9	53.1 ± 10.5^*	3 ± 1.7	0.1 ± 0.1	58.3 ± 11.1
	54	1.9 ± 0.6	55 ± 5.6^**	0.5 ± 0.3	0.3 ± 0.2	57.8 ± 5.6^*
	162	6.5 ± 1.8	57.2 ± 10.2^**	0 ± 0	0.5 ± 0.2	64.2 ± 11.2^*
P90	162	20.4 ± 6.9^**	78.9 ± 12.4^**	0 ± 0	3.9 ± 1.5	103.2 ± 17.2^**
Day 90
VC	0	0.3 ± 0.1	43.3 ± 5	0 ± 0	0.1 ± 0	43.6 ± 5
GO	18	0.9 ± 0.2	43.7 ± 4.9	0 ± 0	0.4 ± 0.1	45 ± 5.1
rGO	18	3.2 ± 1.2^**	37.3 ± 4.5	0.2 ± 0.1	0.4 ± 0.1	41.2 ± 5.7
	54	9.5 ± 3.8^***	40.4 ± 7.5	0.1 ± 0.1	0.7 ± 0.3	50.6 ± 7.2
	162	7 ± 1.6^***	48.9 ± 4	0 ± 0	0.4 ± 0.2	56.3 ± 4.6
P90	162	19.2 ± 4.9^***	62.6 ± 5.5^*	0.2 ± 0.1	4.2 ± 0.9	86.1 ± 9.5^***

Mean ± SEM (n = 7–8).

^a Statistical analysis was performed on neutrophils, macrophages and total cells.

*, ** and ***: Statistically significantly different from corresponding VC at level p < 0.05, p < 0.01, p < 0.001 level, respectively.

^#: Statistically significantly different from corresponding rGO group at level p <0.001.

Table 2. BAL fluid cell counts (x103) (mean ±SEM) in mice at day 1, 3, 28 and 90 post exposure to VC (0.1% TW80), GO, rGO or Printex90 at doses 0, 18, 54 or 162 μg/mouse (n = 7–8).

Table 2. BAL fluid cell counts (x10³) (mean ±SEM) in mice at day 1, 3, 28 and 90 post exposure to VC (0.1% TW80), GO, rGO or Printex90 at doses 0, 18, 54 or 162 μg/mouse (n = 7–8).