Table 3. mRNA expression level of Saa3 and Saa1 in lung and liver of mice 1, 3, 28 and 90 days post exposure to VC, GO, rGO or P90 at doses 0, 18, 54 or 162 μg/mouse.
| Day 1 | Day 3 | Day 28 | Day 90 | ||
|---|---|---|---|---|---|
| Dose | Saa3 mRNA | ||||
| VC | 0 | 389 ± 335 | 96 ± 50 | 60 ± 15 | 74 ± 26 |
| GO | 18 | 50159 ± 11214 (128.9)*** | 2436 ± 1706 (25.4)*** | 80 ± 27 (1.3) | 226 ± 125 (3.1) |
| 54 | 19795 ± 3443 (50.9)*** | 3631 ± 968 (37.8)*** | - | - | |
| 162 | 4016 ± 685 (10.3)*** | 5367 ± 1379 (55.9)*** | - | - | |
| rGO | 18 | 87 ± 19 (0.2) | 68 ± 24 (0.7) | 131 ± 90 (2.2) | 44 ± 6 (0.6) |
| 54 | 135 ± 25 (0.3) | 119 ± 19 (1.2) | 174 ± 72 (2.9) | 152 ± 90 (2.1) | |
| 162 | 441 ± 117 (1.1) | 204 ± 24 (2.1)* | 70 ± 11 (1.2) | 102 ± 14 (1.4) | |
| P90 | 162 | 17803 ± 1023 (45.8)*** | 2459 ± 828 (25.6)*** | 2036 ± 758 (33.9)*** | 1044 ± 274 (14.1)*** |
| Saa1 mRNA | |||||
| VC | 0 | 1624 ± 927 | 377 ± 41 | 717 ± 58 | 689 ± 159 |
| GO | 18 | 51404 ± 9158 (31.7)* | 403 ± 38 (1.1) | 506 ± 124 (0.7) | 906 ± 113 (1.3) |
| 54 | 5097 ± 2849 (3.1) | 2102 ± 833 (5.6) | - | - | |
| 162 | 2824 ± 1063 (1.7) | 21287 ± 9518 (56.5)*** | - | - | |
| rGO | 18 | 1057 ± 327 (0.7) | 373 ± 53 (1) | 869 ± 280 (1.2) | 973 ± 416 (1.4) |
| 54 | 678 ± 68 (0.4) | 294 ± 42 (0.8) | 1001 ± 399 (1.4) | 886 ± 175 (1.3) | |
| 162 | 1839 ± 753 (1.1) | 340 ± 36 (0.9) | 964 ± 377 (1.3) | 546 ± 124 (0.8) | |
| P90 | 162 | 16956 ± 3334 (10.4)*** | 649 ± 130 (1.7) | 840 ± 113 (1.2) | 1963 ± 1603 (2.8) |
Mean ± SEM (fold change), n = 7–8
mRNA was normalized to 18S rRNA and multiplied by 107.
*, **, ***: statistically significantly different from corresponding VC at level p < 0.05, p < 0.01, p < 0.001, respectively.