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. 2016 Jan 1;37(4):1374–1385. doi: 10.1177/0271678X16655550

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Figure 5. — Paravascular distribution of CSF tracer within the brain parenchyma. In contrast to the cortex, tracer was found to penetrate into the parenchyma along vessels on the ventral side of the brain. Sections were stained for laminin (panel A; pink) or myosin heavy chain (panel B; red) and cell nuclei (blue). Tracer is shown in green. Panels A, B: adjacent slides from the same specimen. Panel A was stained for laminin, while panel B was stained for myosin heavy chain, to discriminate arteries (a) from veins (v). This revealed that tracer signal was confined to arteries only. Panels C and D show a longitudinal section of a small artery. The tracer is present around the artery and is located just outside the SMC layer. EC: endothelial cell; SMC: smooth muscle cell. Panels A and B: scale bar 100 µm; Panels C and D: scale bar 10 µm.