Figure 4.

Antibiotics treatment can partially rescue periodontal defects in double knockout (dKO) mice. (a) Representative radiographs reveal restoration (Res) of alveolar bone loss in dKO mice. (b) Micro–computed tomography data show a restoration of alveolar bone, especially in maintaining the height of alveolar crest. (c) Sirius red staining imaged by polarized microscopy is shown for the 3 groups (control [CTR], dKO, and Res mice treated with antibiotics and chlorhexidine gluconate). (d) Restoration of periodontal ligament (PDL) fiber morphology, as detected by periostin immunostaining. (e) Recovery of dentin matrix protein 1 (DMP1) levels in antibiotics-treated mice, as detected by immunostaining. CEJ, cementum-enamel junction.