Figure 4.

Secreted frizzled-related protein 5 (sFRP5) inhibits interleukin (IL)–8 induction by Wnt5a and Porphyromonas gingivalis lipopolysaccharide (Pg-LPS) in human gingival epithelial cells (HGECs). HGECs were treated with medium only or with the indicated molecules or combinations thereof (Pg-LPS, 0.1 µg/mL; Wnt5a, 0.3 µg/mL /mL; sFRP5, 0.5 µg/mL) for 12 h (to assess IL-8 gene expression by quantitative polymerase chain reaction) or 24 h (to assess IL-8 protein release in culture supernatants by enzyme-linked immunosorbent assay). Data are means ± SD (n = 5 sets of cultures). *P < 0.01 between indicated groups; •P < 0.01 compared to untreated (medium only).