Figure 5.

Immunohistochemical analysis. (A) Human mitochondria-positive cells were identified in an in vitro cultured recell-dTB construct. (B) Only a few human cells were detected in 3-mo recell-dTB implants. (C) Regenerated dentin exhibited positive dentin sialophosphoprotein expression (arrows). (D) Regenerated enamel exhibited positive amelogenin expression (arrows). Scale bar = 50 µm in A to D; 1 mm in the boxed areas in B to D. d, dentin; dTB, decellularized tooth bud; e, enamel; od, odontoblast.