Table 1. Primers used for qPCR analysis.
Gene | Accession code | Forward primer | Reverse primer | Product length (bp) |
---|---|---|---|---|
GAPDH | NM_008084 | GGTGGTGAAGCAGGCATCT | GAAGGTGGAAGAGTGGGAGTT | 106 |
PPHcrt | NM_010410 | AACCCATCTTCTATCCTTGTC | CGTCTTTATTGCCATTTACC | 83 |
PPMCH | NM_029971.2 | GAACACAGGCTCCAAACA | GCATTCTGAACTCCATTCTC | 120 |
Abbreviations: GAPDH, glyceraldehyde-3-phosphate dehydrogenase; PPHcrt, prepro hypocretin/orexin; PPMCH, prepro melanin concentrating hormone