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. 2017 Jun 1;12(6):e0178573. doi: 10.1371/journal.pone.0178573

Table 1. Primers used for qPCR analysis.

Gene Accession code Forward primer Reverse primer Product length (bp)
GAPDH NM_008084 GGTGGTGAAGCAGGCATCT GAAGGTGGAAGAGTGGGAGTT 106
PPHcrt NM_010410 AACCCATCTTCTATCCTTGTC CGTCTTTATTGCCATTTACC 83
PPMCH NM_029971.2 GAACACAGGCTCCAAACA GCATTCTGAACTCCATTCTC 120

Abbreviations: GAPDH, glyceraldehyde-3-phosphate dehydrogenase; PPHcrt, prepro hypocretin/orexin; PPMCH, prepro melanin concentrating hormone