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(A) adgrl4 genomic locus, TALEN target site in exon 2 of the adgrl4 gene and a stable mutant line (Δ5) that was analyzed. (B) PCR analysis of adgrl4 mRNA transcripts in 10 to 15 pooled embryo samples from control (ctrl), adgrl4 Δ5+/-, and adgrl4 Δ5-/- fishes. (C) Schematic representation of the different transcripts recovered from the bands (white numbers) in B.
