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. 2017 Jun 1;12(6):e0178748. doi: 10.1371/journal.pone.0178748

Fig 6. CAV1-overexpression downregulates the protein expression levels of lipid metabolism genes in FFA-stimulated L02 cells and AML12 cells.

Fig 6

L02 cells were transfected with adenovirus recombination plasmids containing full-length human CAV1 DNA to overexpress CAV1 (Ad-CAV1), while AML12 hepatocytes were transfected with a lentivirus-constructed system containing mouse full-length CAV1 DNA to stably overexpress CAV1 (Lenti-CAV1). Cells were challenged with 1 mM FFA for 48 h. The protein expression levels of fatty acid metabolism genes, including SREBP1, FASN and ACC, were determined by western blot and quantified with GAPDH as a loading control in L02 cells (A) and AML12 cells (B). pSREBP1 and mSREBP1 denote the precursor and mature forms of SREBP1, respectively. The results represent data from 3 independent experiments.