Fig 3. Impact of calcium on ATP hydrolysis.
Rat aortic rings were incubated ex vivo in sHBSS (supplemented with 1 mmol/L magnesium) and with 1 mmol/L calcium (A) or without calcium (B). Hydrolysis of 1 μmol/L ATP (and 10 μCi/mL [α32P]ATP as radiotracer) showed ADP, AMP and α-Pi production in the indicated times, following separation by thin layer chromatography as described in the methods section. Results are means ± SEM of two independent experiments, with a total of 6 rings per condition (6 different rats). The same ring was used to analyze the hydrolysis of ATP in the absence or presence of calcium.