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. 2017 Jun 1;12(6):e0178872. doi: 10.1371/journal.pone.0178872

Fig 5. Impact of calcium and magnesium on aortic wall calcification.

Fig 5

Rat aortic rings were incubated ex vivo in DMEM supplemented with the indicated concentration of magnesium and calcium. The medium was replaced every day and contained 45-calcium as a radiotracer. After 7 days of incubation, aortic rings were dried and radioactivity was measured by liquid scintillation counting. Results are represented as means ± SEM of two independent experiments, with a total of 14 rings per condition and 11 rats in total (see S5 Table). One-way ANOVA and Tukey’s multiple comparison test were used for statistical analysis. *p <0.05; ***p <0.001.