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. 2017 May 17;13(5):e1006785. doi: 10.1371/journal.pgen.1006785

Fig 5. Numb mediates the effects of ban in the feedback regulation of N activity and CSC-like NB proliferation.

Fig 5

(A) Genetic interaction between ban and numb on the expression of N activity reporter E(spl)m g-GFP. The effect of ban-sp (II) OE driven by 1407-Gal4 on N activity was rescued by numb-RNAi. (B) Quantification of data from A. *, p<0.05 vs. 1407>ban-sp (II); n = 10–14 type I NBs/genotype. (C) The effect of ban-sp in blocking N-induced brain tumor formation is sensitive to the gene dosage of numb but not pros or brat. N-V5 (II) and N-V5 (III) stand for N-V5 transgenes on the 2nd or 3rd chromosomes, respectively. (D) Quantification of data from C. **, p<0.00001 comparing 1407>ban-sp (III) with and without numb-IR in N-V5 background; *, p<0.0005 comparing 1407>ban-sp (III) with or without removing one copy of numb, in N-V5 (II) or N-V5 (III) OE background. n = 5–10 brain samples. (E) Myc OE, but not CycE OE, rescued ban-sp effect in blocking N-induced brain tumor formation. Data is quantified using images from C. *, p<0.00001 comparing 1407>N-V5; ban-sp without Tg expression (w-) vs. with Tg expression (Myc or CycE). Scale bars: A, 20 μm; C, 50 μm.