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. 2017 Jun 1;12(6):e0179107. doi: 10.1371/journal.pone.0179107

Fig 6. Analysis of nNOS immunohistochemistry.

Fig 6

(A) Photomicrograph of nNOS immunostaining of the corpus of rat stomach. Arrows and arrowheads indicate the nNOS-positive nerve fibers and neuronal ganglion, respectively (x200 magnification). (B) Comparison of the nNOS positive area (n = 6 per group). The proportion of the nNOS immunoreactive area decreased with age. Each bar represents the mean ± SE. SMB, submucosal border; MP, myenteric plexus; CM, circular muscle; LM, longitudinal muscle.*P < 0.05 compared with 6-wk-old rats; †P < 0.05 compared with 31-wk-old rats; ‡P < 0.05 compared with 74-wk-old rats.