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. 2017 Jun 1;13(6):e1006383. doi: 10.1371/journal.ppat.1006383

Fig 5. Increased C. trachomatis replication is associated with elevated inflammasome activation.

Fig 5

(A) IL-1β maturation in response to C. trachomatis infection, analysed by western blot of cell lysates in mDC obtained from healthy donors (HD) or CGD donors (CGD). Data representative of three-independent donors in each patient group. (B) IL-1β secretion analysed by ELISA from mDC obtained from healthy donors or CGD patient donors infected with C. trachomatis (CT) for 24hrs. Data represents the mean from mDC obtained from seven-donors from each group, error bars indicate ±SEM **p = <0.01. (C) IL-1β maturation in response to C. trachomatis infection, analysed by western blot of cell lysates and supernatants from wild-type (Cybb+/+) or NADPH oxidase deficient (Cybb-/-) BMDM. Data represents the response from BMDM obtained from two-individual mice. (D) IL-1β mRNA expression relative to HPRT analysed by qRT-PCR of RNA obtained from wild-type (Cybb+/+) or NADPH oxidase deficient (Cybb-/-) BMDM following infection with C. trachomatis for 6-hours. Data represent the mean from three-individual mice, error bars indicate ±SEM, ns indicates no significant difference. (E) IL-1β secretion analysed by ELISA or (F) Cell death analysed by LDH release from BMDM obtained from wild-type (Cybb+/+) or NADPH oxidase deficient (Cybb-/-) mice following stimulation with gamma-attenuated C. trachomatis (γ-CT), live C. trachomatis (CT) or LPS/ATP for 24hrs. Data represent the mean from 12-individual mice (CT) or six-individual mice (control, γ-CT and LPS/ATP), error bars indicate ±SEM. **p = <0.01, ***p = <0.001, ns indicates no significant difference.