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. 2017 Jun 1;13(6):e1006383. doi: 10.1371/journal.ppat.1006383

Fig 6. Type-1 interferon signalling regulates C. trachomatis inflammasome activation.

Fig 6

(A) Induction of IFNβ mRNA expression in wild-type BMDM analysed by quantitative RT-PCR stimulated with gamma attenuated C. trachomatis (γ-CT) or live C. trachomatis (CT) for 8hrs. (B) Induction of IFNβ mRNA expression in wild-type BMDM (Cybb+/+) or NADPH oxidase deficient BMDM (Cybb-/-) in response to infection with live C. trachomatis for 8hrs. Data represent the mean from BMDM obtained from three-individual mice in each group, error bars indicate SEM **p = <0.01, ***p = <0.001. (C) STAT-1 phosphorylation analysed by western blot from BMDM stimulated with 100U/ml IFNβ for indicated times in the presence of isotype (IgG) or IFNAR blocking antibody (α-IFNAR). (D) IL-1β secretion analysed by ELISA of supernatants from wild-type BMDM infected with C. trachomatis (CT) or stimulated with LPS/ATP in the presence of an IFNAR blocking antibody (αIFNAR) or isotype control (isotype IgG) for 24hrs. Data represent the mean from BMDM obtained from nine-individual mice, error bars indicate ±SEM ***p = <0.001. (E) Caspase-11 expression and IL-1β maturation analysed by western blot of lysates and supernatants from wild-type BMDM in response to C. trachomatis infection in the presence of an IFNAR blocking antibody (αIFNAR) or isotype control (isotype IgG) for 24hrs. (F) Cell death analysed by LDH release from wild-type BMDM in response to C. trachomatis infection (CT) in the presence of an IFNAR blocking antibody (αIFNAR) or isotype control (isotype IgG) for 24hrs. Data represent the mean from BMDM obtained from nine-individual mice, error bars indicate ±SEM **p = <0.01.