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. 2017 Jun 1;12(6):e0178391. doi: 10.1371/journal.pone.0178391

Fig 3. Adult and adolescent expression of dihydropyrimidinase-like-2 (DPYSL2).

Fig 3

(A) Representative 3D plot of DPYSL2 expression in adult (left) and adolescent (right) mice for Spot #20. (B) Standardized abundance (log) of DPYSL2 demonstrating higher expression in adults versus adolescents. (C) Representative gel image of a Western blot for DPYSL2 expression to confirm 2D-DIGE changes. Both resulting bands were quantified. (D) Quantification of Western blot results, confirming reduced expression of DPYSL2 (normalized to actin) in adolescents as compared to adults. (E) Top, representative gel images for each brain region; bottom, quantification of Western blots for each brain region. Adults show increased expression of DPYSL2 in dStr, NAc, MC and Amy. No significant age differences were observed in the VTA (p>0.05). Data were expressed as percent change from mean adult within the same blot and graphed as mean ± SEM. (* indicates p≤0.05, *** indicates p≤0.001).