Figure 4.
Compound pharmacology. (A) Cytotoxicity was measured by Alamar Blue assay in FRT cells incubated for 8 h with 10 μM 6k or 12, with 33% DMSO as positive control (mean ± SEM, n = 8). (B) In vitro metabolic stability. Compounds at 5 μM were incubated for indicated times with 1 mg/mL hepatic microsomes in the presence of NADPH and parent compound assayed by LC/MS (mean ± SEM, n = 3). LC/MS profile of 12 is shown on the right with elution time on the x-axis for incubation times of 0, 15, and 60 min.