Table 2.

The Effect of Compounds (13, 17a–e, 21a–c) on Hb Affinity for Oxygen Using Normal Whole Blood ^a.

Comp.	Name	R1	R2	N	Mean P50 b	∆P50 c
Ctr	DMSO d				38.3 ± 0.0	0.0
13	KAUS-25	Cl	H	1	39.7 ± 0.3	1.4 ± 0.3
17a	KAUS-19	Cl	Cl	1	39.2 ± 0.2	0.9 ± 0.2
17b	KAUS-22	H	Cl	1	41.3 ± 0.05	2.9 ± 0.05
21c	KAUS-26	Cl	H	2	39.7 ± 0.1	1.4 ± 0.1
21a	KAUS-20	Cl	Cl	2	38.9 ± 0.3	0.7 ± 0.3
21b	KAUS-23	H	Cl	2	40.8 ± 0.02	2.5 ± 0.02
17e	KAUS-27	Cl	H	3	39.1 ± 0.3	0.8 ± 0.3
17c	KAUS-21	Cl	Cl	3	39.6 ± 0.6	1.3 ± 0.6
17d	KAUS-24	H	Cl	3	41.7 ± 0.3	3.4 ± 0.3

^a The results are the means of two or three measurements. ^b P₅₀ is the oxygen pressure at which normal RBC (22% hematocrit) is 50% saturated with oxygen. ^c ΔP₅₀ is P₅₀ of compound treated cells—P₅₀ of control. ^d The final concentration of DMSO was <2% in all samples.