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Nitrate binding at βHis97 of bis-ligated T-state HbNO. (A) Difference electron density (Fo-Fc) map of bound NO3 (before NO3 was built into the model), contoured at 3.5σ. (B) Final 2Fo-Fc map of bound NO3 (before NO3 was built into the model), contoured at 1.2σ. The maps were superimposed with the final refined model. (C) Comparison of the NO3 binding site of bis-ligated T-state HbNO (yellow) and native T-state deoxyHb (blue).
