. Author manuscript; available in PMC: 2017 Jun 1.

Published in final edited form as: Biochemistry. 2015 Dec 3;54(49):7178–7192. doi: 10.1021/acs.biochem.5b01074

Table 2.

Effects of compounds on Hb oxygen affinity with normal whole blood (30% hematocrit).

Compound	ΔP₅₀ (mmHg)
Compound	1h	2h	4h	8h
RSR13	25.4 ± 4.5	32.1 ± 5.8	30.4 ± 3.1	26.1 ± 2.7
DD-1	10.0 ± 0.5	15.2 ± 0.8	13.6 ± 0.5	11.5 ± 3.1
DD-2	6.3 ± 0.9	9.5 ± 1.7	13.0 ± 0.4	20.7 ± 0.8
DD-3	4.9 ± 0.9	10.4 ± 0.4	20.2 ± 2.7	21.7 ± 5.6

The experiments performed in duplicate or triplicate. ΔP₅₀ = (P₅₀ control – P₅₀ sample). All compounds were solubilized in DMSO (2%). Control experiments without test compound also contain 2% DMSO