Figure 2. t-SNE maps of epithelial cells in ileum reveal tuft cell heterogeneity.

(A) t-SNE maps of all epithelial cells in a mouse ileum (n = 129,379) reveal discrete localization of differentiated cell types. DCLK1 is constrained to a single “island,” while other tuft cell markers are expressed in other differentiated cell types. (B) Isolation of the tuft cell “island” demonstrates uniform DCLK1 expression and heterogeneous patterns of expression of other tuft cell markers.