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. 2017 Jun 2;2(11):e93166. doi: 10.1172/jci.insight.93166

Figure 2. Mouse scBAT continues to grow after birth.

Figure 2

(A) Diagram showing the anatomical location of scBAT in adult mice (ventral view). (B) Representative images showing the anatomical location of scBAT in 8-, 24-, and 52-week-old mice. Top row: Lower-magnification images of the ventral neck. sg, salivary gland; tr, trachea; jv, external jugular vein. scBAT is outlined by the black dotted line. Bottom row: Higher-magnification images of the ventral neck showing close-up images of scBAT. *scBAT. Scale bars: 1,000 μm. (C) Representative image of the ventral neck from an 8-week-old mouse showing the sternocleidomastoid muscle (#) after scBAT was removed. Scale bars: 1,000 μm. (D) Representative images of iBAT and scBAT isolated from an 8-week-old mouse. Arrows point to the part of the scBAT that is located behind the external jugular vein. Scale bars: 1,000 μm. (E) Body weight, iBAT and scBAT mass, and weight/mass ratio of iBAT and scBAT in 3-, 8-, 26-, and 52-week-old male mice. n = 5–6. (F) Representative H&E-stained sections of iBAT, scBAT, iWAT (inguinal WAT), and eWAT (epididymal WAT) isolated from 8-week-old male mouse. n = 3. Scale bars: 50 μm. (G) Lipid droplet size distribution in iBAT, scBAT, iWAT, and eWAT from 8-week-old male mice. Percentages for different lipid droplet sizes were measured in 4–8 randomly selected H&E-stained sections from 3 mice. (H) Relative mRNA expression of BAT selective markers in iBAT, scBAT, iWAT, and eWAT isolated from 8-week-old male mice. Data are presented as mean ± SEM. n = 4–5. ****P < 0.0001, ***P < 0.001, **P < 0.01, *P < 0.05. One-way ANOVA.