Figure 4. The β3-adrenergic pathway mediates thermoactivation of scBAT in mice.
(A) Effect of CL316243 (CL) treatment on relative expression of lipid, glucose uptake, and thermogenesis marker mRNA in iBAT and scBAT in 12-week-old mice. Data are presented as mean ± SEM. n = 4. ****P < 0.0001, ***P < 0.001, **P < 0.01, * P < 0.05; ns, nonsignificant (P > 0.05). Two-tailed t test for 2 groups and 1-way ANOVA for 4 groups. (B) Western blot image of UCP1 in iBAT and scBAT from 12-week-old mice treated with or without CL316243. β-Actin was used as protein loading control. n = 3.