Figure 2. Expression of allergen in DC prevents airway inflammation.
(A–E and H) DC.OVA or nontransgenic littermates (non-Tg) were sensitized with OVA323-339/alum (OVAp/alum; d0, d14), were sham-sensitized with PBS/alum (sham-sens), or were left unsensitized (un-sens) and i.n. challenged with OVA daily (days 11–14 and 19–24 after sensitization). One day after the last i.n. challenge, mice were euthanized for analysis. Cytokine production in response to OVA323-339 restimulation of mediastinal lymph nodes (MLNC) (A–C) and spleen (D and E) was determined by ELISA. Eosinophil infiltrate in bronchoalveolar lavage fluid (BALF) was determined by flow cytometry (H). (F and G) DC.OVA or non-Tg littermates were sensitized with OVA/alum (d0, d14) or sham-sensitized with PBS/alum (sham-sens) and i.n. challenged with OVA (days 11–14 and 19–24 after sensitization). One day after the last i.n. challenge, mice were euthanized for analysis. IL-4–secreting cells (IL-4 SFU) were enumerated in mediastinal lymph node cells (MLNC) (F) and spleen (G) by ELISpot. Data show values for individual mice pooled from 3–4 experiments, with box and whisker plots showing median, quartiles, and range. ANOVA/Newman-Keuls post-test. Dotted line denotes detection limit of ELISA.