Figure 5. BM transfer terminates Th2 responses.

(A–G) Nontransgenic BALB/c mice were sensitized with OVA_323-339/alum i.p. or sham-sensitized with PBS/alum (un-sens) twice, 14 days apart. One week after second sensitization, mice were irradiated (300 cGy) and DC.OVA or non-Tg BM transferred i.v. Four weeks after BMT, mice were i.n. challenged daily with OVA (5 consecutive days i.n., 4 days rest, 5 consecutive days i.n.). One day after the last i.n. challenge, mice were euthanized for analysis. Cytokine production in response to OVA_323-339 restimulation of mediastinal lymph node cells (MLNC) (A–C) was determined by ELISA, and eosinophils in bronchoalveolar lavage fluid (BALF) was determined by flow cytometry (D). Lungs were collected and H&E or periodic acid–Schiff (PAS) stained (E), and images were analyzed to define cellular infiltrate (F) and mucus-filled goblet cell frequency (G). Scale bars (E) depict 1 mm (low-power) and 200 μm (high-power inset). Data are representative micrographs from 6 mice per group taken from 2 experiments or represent values for individual mice, with box and whisker plots showing median, quartiles, and range pooled from 2 independent experiments. ANOVA/Newman-Keuls post-test.