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. 2017 Jun 2;2(11):e85742. doi: 10.1172/jci.insight.85742

Figure 6. Inactivation of Th2 responses by BM transfer is specific to the expressed allergen.

Figure 6

(A and B) Non-Tg mice were sensitized with OVA323-339/ryegrass pollen extract (RGP)/alum i.p. twice 14 days apart or left unsensitized (un-sens), and 1 week later, they were irradiated (300 cGy) and DC.OVA or non-Tg BM transferred i.v. Four weeks after BMT, mice were i.n. challenged with RGP daily (5 consecutive days i.n., 4 days rest, 5 consecutive days i.n.). One day after the last i.n. challenge, mice were euthanized for analysis, cytokine production in response to RGP stimulation was measured by ELISA (A and B), and eosinophils in bronchoalveolar lavage fluid (BALF) was determined by flow cytometry (C). Lungs were collected and stained, and cellular infiltrate (D) and mucus-filled goblet cell frequency (E) were determined. Data represent values for individual mice pooled from 2 independent experiments. Box and whisker plots show median, quartiles, and range. ANOVA/Newman-Keuls post-test.