Figure 4. Immunophenotype of breast cancer (BC) T_FHX13 tumor-infiltrating lymphocytes (TIL) in comparison with their counterparts from blood and tonsils.

Flow cytometric analyses showing: (A) T_FH and Treg/activation marker expression on CXCL13⁺CD4⁺ T cells in fresh tissue homogenates from a representative tonsil and BC; (B) proliferation and activation markers associated with CXCR5⁺CD4⁺ or CXCR5⁺CD8⁺ TIL; (C) CXCR5 expression on tonsillar CD4⁺ T cells indicating (top) ± primary tumor supernatant (SN; representative data shown for 3 tonsils using 12 BC SN) and (bottom) ± CXCL13 (representative data shown for 3 tonsils); (D) CXCL13 expression after stimulation of healthy donor peripheral blood (D-PB) with αCD3/αCD28 Ab or Staphylococcal enterotoxin B (SEB); and (E) expression of the markers in A plus T-bet on stimulated D-PB CD4⁺ T cells.