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. Author manuscript; available in PMC: 2017 Jun 1.
Published in final edited form as: Inorg Chem. 2015 Feb 10;54(5):2278–2283. doi: 10.1021/ic502881q

Table 1.

Observed thermodynamic values from the ITC titration of Fe2+ acetate into TauD

25 mM Buffer (pH 7.4) n K ΔHobs (kcal/mol) ΔHionization (kcal/mol) ΔG (kcal/mol) −TΔSobs (kcal/mol) KFe-buffer
MOPS 0.78 (± 0.13) 5.4 (± 0.7) × 105 −2.2 (± 0.3) −5.04 −7.8 (± 0.1) −5.7 (± 0.4) 42.0 (± 5)
HEPES 0.85 (± 0.13) 3.3 (± 0.6) × 105 −1.9 (± 0.5) −4.86 −7.5 (± 0.1) −5.6 (± 0.4) 72.5 (± 14)
ACES 0.94 (± 0.07) 1.0 (± 0.7) × 107 −3.9 (± 0.8) −7.27 −9.2 (± 1.0) −5.3(± 1.5) 19.7 (± 13)

Values are the average of 2–4 replicates and the errors associated with the values are one standard deviation from the mean.