. Author manuscript; available in PMC: 2017 Jun 1.

Published in final edited form as: Inorg Chem. 2015 Feb 10;54(5):2278–2283. doi: 10.1021/ic502881q

Table 2.

The thermodynamic cycle for Fe²⁺ binding to TauD in MOPS buffer

Reaction	Coefficient	ΔH (kcal/mol)
Fe²⁺-MOPS + TauD-H⁺_0.9 Fe²⁺-TauD + MOPS-H⁺_0.9 + 0.1 MOPS		−2.17^a
Fe²⁺-MOPS Fe²⁺ + MOPS	1.0	1.83^b
TauD-H⁺ TauD + H⁺	0.91	13.34^c
MOPS + H⁺ MOPS-H⁺	0.91	−5.04^d
Fe²⁺ + TauD Fe²⁺-TauD	1.0	−11.55

Calculated from control experiments of EDTA chelation of Fe²⁺ in MOPS buffer.

Represents the ionization of water within the Fe²⁺-TauD complex. Value is taken from reference.²⁶

Value taken from reference.³⁰