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The average enthalpy values in each buffer for Fe2+ binding to TauD
| 25 mM Buffer, pH 7.4 |
ΔHFe-TauD (kcal/mol) |
|---|---|
| MOPS | −11.46 (± 0.01) |
| HEPES | −11.53 (± 0.49) |
| ACES | −11.94 (± 0.83) |
| Average | −11.64 (± 0.25) |
Values were calculated based on the thermodynamic cycle for the binding reaction in the respective buffering system. Values were averaged and the error represents one standard deviation from the mean.
