Fig. 3.
Effect of P2Y2R gene deletion on VCAM-1 in different regions of the mouse aorta. A, Real-time PCR analysis of VCAM-1 mRNA levels in the aortic sinus of wild-type (n = 4) and P2Y2R−/− (n = 3) mice. P < 0.05. B, representative en face immunostaining for VCAM-1 expression in the arch and descending thoracic aorta in P2Y2R−/− mice. No VCAM-1-positive cell was detected in either part of the aorta. Endothelial cell nuclei are in shown blue. Scale bar, 20 μm. P < 0.05. n = 15 visual fields from 4 different mice.