. Author manuscript; available in PMC: 2018 Apr 20.

Published in final edited form as: Cell Chem Biol. 2017 Mar 16;24(4):443–457.e6. doi: 10.1016/j.chembiol.2017.02.013

Table 1.

Summary of Rpn1 interactions

Analyte^*	Ub (NH)	K48-Ub₂(Dist,NH)	Rpn1 (CH3)	K63-Ub₂ (Dist,NH)	Dsk2 UBL (NH)	Ubp6 UBL (NH)	Rub1 (NH)
ligand	Rpn1^PC1	Rpn1^PC1	K48-Ub₂	Rpn1^PC1	Rpn1^PC1	Rpn1^PC1	Rpn1^PC1
K_D (μM)	214±68	116±30	112±29	103±59	22±12	40±31	280±20

Titration of Rpn1 to 15N-Rad23 caused severe signal broadening in amide signals that precluded accurate determination of signal shifts for K_D determination. The observation of signal broadening indicates intermediate or slow exchange regime, likely due to slow off-rates.