TABLE III.

Quantitative RT-PCR Mean C_T (+/−SD) Values and Fold Change^a of Selected Probes From the Gene Expression Microarrays Comparing Gene Expression in Brain of Normal Littermates to PWS IC Deletion Mice

Group	Pomc*	Mc5r**	AK013560	BB314814	BB182944
Control littermates	21.4 (0.5)	24.7 (0.3)	22.5 (0.9)	38.2 (0.7)	32.5 (0.7)
PWS IC deletion	18.1 (0.6), 9.9-fold change	23.6 (0.5), 2.1-fold change	nd	nd	nd

Significantly different by t-test.

nd, not detected.

^aThe C_T was set at the narrowest portion of the logarithmic phase of amplification in the quantitative RT-PCR reaction. Fold change = 2 |^{Control C}_T ^{− PWS IC C}_T| (e.g., 2|^21.4−18.1| = 2^3.3 = 9.9-fold change).

^*P=0.002.

^**P=0.019.