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. Author manuscript; available in PMC: 2018 Apr 1.
Published in final edited form as: Endocr Relat Cancer. 2017 Apr;24(4):157–170. doi: 10.1530/ERC-16-0138

Figure 2.

Figure 2

Mouse AR-ASO decreases AR expression in stromal cell lines with little effect on proliferation. A, RT-PCR results of mouse AR mRNA levels in mouse NPF (3395) and CAF (2270 & 481) cells treated with the mouse AR-ASO for 24 hours. B, Western blot results of mouse AR expression in untreated NPF3395 and CAF2270 (lane 1) or following 24 hour treatment with 2.5 μM control ASO (lane 2) or AR-ASO (lane 3). Actin served as the loading control. C, Mouse CAF3234 cells were treated with mouse AR-ASO at various concentrations for 1, 4, and 7 days. Mouse AR expression was analyzed by RT-qPCR. Control, control-ASO (10 μM). (*, p<0.05). D, Mouse CAF and NPF cells were treated with 10 μM control-ASO or mouse AR-ASO at indicated concentrations for 24 hours. Proliferation was assessed by MTS assay. Representative results are presented from ≥3 independent replicates.