TABLE 1.

t Test Results for Subjects With TI and TII Deletions

Gene	N	Mean CT Value	SD	Fold Change	P
NIPA1				4.3	.001
TI	8	19.95	0.77
TII	9	17.84	1.30
NIPA2				5.9	.003
TI	8	21.90	1.86
TII	9	19.33	1.16
CYFIP1				4.7	.007
TI	8	24.61	1.77
TII	9	22.37	1.17
GCP5				4.1	.048
TI	8	22.32	2.63
TII	9	20.29	0.98

Gene-expression fold change is calculated as 2^{|TI C_T−TII C_T|}; for example, the fold change between subjects with PWS with TI deletions compared to those with TII deletions for NIPA1 = 2^{|19.95 − 17.84|} − 2^2.11 or 4.3.