Figure 5.

TCDD, but not aspirin induces Tregs in the LP of the colon, concomitant with AhR activation. (A, B) WT mice were orally treated once with 5 μg/kg of TCDD. Three days later, the percentages of Foxp3⁺ cells within the CD3⁺CD4⁺ cell population isolated from the colon LP of mice were analyzed by flow cytometry. (A) Representative plots (left) and quantitative data (right). (B) Cyp1A1 mRNA levels in the colon. (C, D) WT mice were orally treated with aspirin added to drinking water for 2 weeks, the percentages of Foxp3⁺ cells within the CD3⁺CD4⁺ cell population isolated from the colon LP of mice were analyzed by flow cytometry. (C) Representative plots (left) and quantitative data (right). (D) Cyp1A1 mRNA levels in the colon. Error bars represent mean ± SEM, n = 4 mice per group. *P < .05, **P < .01.