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. 2017 Jun 1;7:2675. doi: 10.1038/s41598-017-02860-4

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Divergence in body CG methylation (BCGM) level (a) and expression (b) between duplicated copies of each of the four different categories of duplicates with CG-only methylation in WT rice and the OsMet1-2 null mutant. The y axis in (a) denotes divergence in BCGM levels between duplicated copies of each duplication category. Pairwise comparisons showed significant differences in between-copy BCGM divergence among some (indicated by different small letters) but not all (indicated by the same small letters) of the four duplication categories in WT (ANOVA and Tukey’s honestly significant different (HSD) test, p < 2.42e-14). Significant reduction of between-copy divergence in BCGM was detected in mutant versus WT in all duplication categories (Kolmogorov-Smirnov test, p values < 0.001). The y axis in (b) shows absolute value of fold changes of between-copy expression levels of each duplication category in WT and mutant. Significant reduction of between-copy expression difference in mutant versus WT was detected for all categories of duplicated genes (Kolmogorov-Smirnov test, p values < 0.05).