Figure 4.

Elevated intracellular Ca²⁺ levels in miR-150 deficient CD8⁺ T cells are caused by down-related PMCA activity. (a) Intracellular Ca²⁺ levels in mir-150 ^+/+ or mir-150 ^−/− naïve CD8⁺ T cells in the presence or absence of CRAC inhibitor (2-Aminoethoxydiphenyl borate, 2-APB) (left) and the intracellular Ca²⁺ levels at 10 min incubation (middle). Images are the representatives at 10 min incubation (right). mir-150 ^+/+ or mir-150 ^−/− naïve CD8⁺ T cells were incubated in 2 mM of Ca²⁺ containing medium in the presence or absence of 2-APB for 10 min and measured the intracellular Ca²⁺ levels from the cells. (b) Change in the intracellular Ca²⁺ level by treatment with PMCA inhibitor, La³⁺ (0.3, 0.5 or 1 mM), in mir-150 ^+/+ and mir-150 ^−/− naïve CD8⁺ T cells. mir-150 ^+/+ and mir-150 ^−/− naïve CD8⁺ T cells were cultures with 2 mM of Ca²⁺ containing medium and incubated in the presence or absence of indicated concentration of La³⁺ (up). Dot graph shows intracellular Ca²⁺ level at 15 min incubation (down left). Images are representatives for the intracellular Ca²⁺ level from the CD8⁺ T cells at 15 min incubation (down right). **P < 0.01, ***P < 0.001, ns: not significant. Data are means ± SEM of duplicate triplicate samples from a single experiment and are representative of two independent experiments.