Figure 5.

miR-150 specifically binds to 3′UTR of TMEM20 and down-regulates TMEM20 expression. (a) The expression level of Ca²⁺-associated molecules in mir-150 ^+/+ and mir-150 ^−/− naïve CD8⁺ T cells (left). The relative amount of TMEM20 mRNA in mir-150 ^+/+ and mir-150 ^−/− naïve CD8⁺ T cells (right). Western data are the cropped blot images representing indicated proteins. (b) Co-localization of STIM1, TMEM20 and PMCA in mir-150 ^+/+ and mir-150 ^−/− naïve CD8⁺ T cells (up) and the fluorescence intensity for each molecules on the line (down). Green; PMCA, Red; STIM1, Purple; TMEM20. (c) Post-transcriptional regulation of miR-150 by targeting 3′UTR of TMEM20. miR-150 binding sequences of TMEM20 3′UTR (left up). The level of AANAT protein (left down) and mRNA (right) in NIH3T3 cells co-transfected with plasmid-expressing AANAT reporter containing TMEM20 3′UTR and miR-150 or miR-150 mutant. Western data are the cropped blot images representing indicated proteins. *P < 0.05. Data are means ± SEM of duplicate triplicate samples from a single experiment and are representative of two independent experiments.