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. 2017 Jun 1;7:2738. doi: 10.1038/s41598-017-02885-9

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Adipose tissue (AT) depot weight (A), lipolysis in isolated mature adipocytes (B) and p,p’-DDE levels in AT (concentration, CI; burden, CII), after 12 weeks. Values are represented as mean ± SEM. Statistical analysis with (A,B) two-way ANOVA (main effects: diet, p,p’-DDE exposure and their interaction; p < 0.05), followed by Tukey’s multiple comparison post-hoc test: *p < 0.05 vs St and/or St/DDE; (C) unpaired t test when comparing between treatment groups (St/DDE and HF/DDE) and paired t test when comparing between different ATs from the same treatment group: *p < 0.05. St, standard diet; HF, high fat diet; p,p’-DDE, p,p’-dichlorodiphenyldichloroethylene; vAT, visceral adipose tissue; scAT, subcutaneous adipose tissue; NEFAs, non-esterified fatty acids.