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. 2017 Jun 1;7:2645. doi: 10.1038/s41598-017-02467-9

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Confocal photomicrographs of fatty acid binding protein 3 (FABP3) immunodetection in transzonal projections (TZPs) after 9 h of in vitro maturation (IVM). FABP3 is immunostained using 488 Alexa Fluor (green); TZPs (actin) are stained with Alexa Fluor 647 phalloidin (red); nuclei are stained with DAPI (blue). (A) Photomicrographs of COCs after 9 h of IVM were obtained in 63x objective and (B) in 63x objective zoomed 3.5x. (C) Digital zoom showing FABP3 (arrow) along TZPs (asterisk) in the zona pellucida (OO = oocyte; CC = cumulus cells). A total of 12 COCs in vitro-matured for 9 hours were analysed and showed similar pattern; 6 additional COCs are shown in Supplementary Fig. S4.