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. 2017 Jun 1;7:2608. doi: 10.1038/s41598-017-02566-7

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Figure. 6 — Validation of RNA-Seq data with quantitative real-time reverse transcription PCR (qRT-PCR). (a) Bar plot of qRT-PCR relative expression in Asparagus kiusianus and A. officinalis treated with distilled water (AKC and AOC, respectively) and inoculated with Phomopsis asparagi (AKI and AOI, respectively). (b) Scatter plot correlation between qRT-PCR and RNA-Seq log2 fold changes (Pearson’s correlation coefficient = 0.78). Values represent the mean on three independent biological replicates (n = 3) ± standard deviation (SD). Significance levels are given as: *(P < 0.05) and **(P < 0.01) and according to analysis of variance (ANOVA).