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. 2017 Jun 1;7:2605. doi: 10.1038/s41598-017-02604-4

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Western blot analysis of xenografted immature and adult testes at 1-and 2-wk-post grafting for expression of PI3K/AKT/mTOR pathway proteins. Protein expression in 6-day-old and 10-wk- old donor testes before grafting (T0) are presented as starting material. Representative blot (A) and densitometry analysis of (B) PTEN, (C) PI3Kp85alpha/gamma-pY467/199, (D) AKT, (E) AKT-pSer473, (F) AKT-pTyr308, (G) eNOS, (H) mTOR and (I) mTOR-pSer2448 protein. Y-axis represents intensity of bands relative to GAPDH. Data are presented as mean ± SEM. Bars with different letters are significantly different at P < 0.05.