Figure 4.

Maximal respiration and reserve capacity are increased after 18 h exposure to Exendin-4. (A) Following 18 h of incubation in the presence or absence of 50 nM Exendin-4, BRIN-BD11 cells were subjected to extracellular flux analysis. OCR was determined sequentially after injection of 25 mM glucose followed by a set of mitochondrial inhibitors (2 µM oligomycin, 0.3 µM FCCP and 1 µM each of rotenone and antimycin A) to generate a mitochondrial stress profile. (B–G) Mitochondrial parameters were calculated from the mitochondrial stress profile as described in Methods. (B) Maximal respiration, (C) reserve capacity, (D) mitochondrial ATP turnover, (E) basal respiration, (F) proton leak, (G) non-mitochondrial OCR. Data represent mean ± SEM, n = 3; n.s. = non-significant; *P < 0.05; **P < 0.01.