Table 3.
m/z values ([M+H]+, monoisotopic) of VGF peptides detected in rat brain by RP-high-pressure performance liquid chromatography (HPLC) high-resolution ESI mass spectrometry (MS) and confirmed by MS/MS experiments.
| Position | Experimental [M+H]+ | Modifications | Sequences |
|---|---|---|---|
| 24–54 | 3242.58 (± 0.03) | – | APPGRSDVYPPPLGSEHNGQVAEDAVSRPKD |
| 24–60 | 3868.88 (± 0.04) | – | APPGRSDVYPPPLGSEHNGQVAEDAVSRPKDDSVPEV |
| 180–209 | 3390.67 (± 0.03) | N-Term (Q→pyro D) | QQETAAAETETRTHTLTRVNLESPGPERVW |
| 238–282 | 4836.47 (± 0.05) | – | MSENVPLPETHQFGEGVSSPKTHLGETLTPLSKAYQSLSAPFPKV |
| 285–309 | 2558.31 (± 0.03) | C-Term (Amidated) | LEGSFLGGSEAGERLLQQGLAQVEA |
| 301–329 | 3150.61 (± 0.04) | C-Term (Amidated) | QQGLAQVEAGRRQAEATRQAAAQEERLAD |
| 353–372 | 2461.08 (± 0.03) | – | GLQETQQERENEREEEAEQE |
| 360–372 | 1391.55 (± 0.03) | – | ENEREEEAEQE |
| 487–507 | 2171.21 (± 0.03) | – | KKNAPPEPVPPPRAAPAPTHV |
| 489–507 | 1915.02 (± 0.03) | – | NAPPEPVPPPRAAPAPTHV |
| 587–600 | 1700.83 (± 0.03) | – | RAQEEADAEERRLQ |
| 588–600 | 1544.73 (± 0.03) | – | AQEEADAEERRLQ |
Numbering refers to the sequence of the protein with the signal peptide.