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. 2017 Jun 1;7:2653. doi: 10.1038/s41598-017-02517-2

Figure 2.

Figure 2

ArtA and pentameric ArtB subunits of ArtAB. (A) Separation of ArtA and ArtB by Mono-Q anion exchange chromatography. The dashed line shows the increasing salt gradient (0 to 0.5 M NaCl in 30 mM Tris-HCl, pH 8.8). (B) SDS-PAGE analysis of the protein content of the ArtA and ArtB chromatographic fractions obtained by Mono-Q anion exchange chromatography. The gel was stained using a silver staining kit. These along with purified ArtAB were separated by electrophoresis in the presence or absence of 100 mM DTT. Molecular weight standards (kDa) are indicated on the left; positions of the toxin subunits are indicated on the right. (C) Schematic illustration of ArtAB subunit structure.