Table 1.
List of proteomic studies focused on drought stress in cereal crops.
| Cultivar | Plant material | Method | Protein identification (TI: total identification, DRP: Drought responsive proteins) | Up-regulated protein candidates | References |
|---|---|---|---|---|---|
| WHEAT | |||||
| Triticum aestivum L. Arvand, Khazar-1, and Kelk Afghani | Seeds | 2-DE, MALDI-TOF/TOF | TI: 121 DRP: 57 |
Trx h, 1-Cys peroxiredoxin, GST, PDI, LEA, sHSP17, HSP70 | Hajheidari et al., 2007 |
| Durum wheat cv. Ofanto | Leaves | 2-DE-PAGE, MALDI-TOF-MS | DRP: 36 | Carbonic anhydrase, RubisCO LSU | Caruso et al., 2009 |
| Triticum aestivum L. cv. China-108, cv. Yennon-78, cv. Norin-61,cv. Kantou-107 | Grains | MALDI-TOF-MS | DRP: 33 | ABA responsive proteins, LEA, cys-peroxiredoxin, elf proteins, cyclin-dependent kinase like, MYB, lipid transfer proteins and WRKY | Kamal et al., 2010 |
| Triticum aestivum L. cv. Shanrong No. 3, cv. Jinan 177 | Roots and leaves | MALDI-TOF/TOF-MS | DRP: 93 Roots, DRP: 65 leaves | G protein -subunit-like protein, Rubisco LSU, Serine/threonine kinase, GTP-binding protein, Glutathione transferase F4 | Peng et al., 2009 |
| Australian wheats cv. Kukri, cv. excalibur, cv. RAC875 | Leaves | nanoLC-MS/MS | TI: 1299 | CAT, Cu/Zn-SOD, Mn-SOD | Ford et al., 2011 |
| Triticum aestivum L. cv. Nesser, cv. Opata M85 | Roots | nanoLC-MS/MS, iTRAQ | TI: 1656, ABA- Responsive Proteins: 805 | LEA, PP2C, HSP70, HSP90, 14-3-3, G-proteins; V-ATPase; β- expansin, porins | Alvarez et al., 2014 |
| Triticum aestivum L. SERI M 82, SW89.5193/kAu2 | Roots and leaves | 2DE, nano-LC-MS/MS | TI: 237 DRP: 49 |
16.9 kDa HSP,GST, Germin-like protein, 14-3-3 protein, ATP synthase | Faghani et al., 2015 |
| Triticum boeoticum | Roots and leaves | 2DE, MALDI-TOF/TOF | Roots DE: 98, leaves DRP: 85 | UDP-glucose/GDP-mannose dehydrogenase, transketolase, transaldolase-like protein, ribulose-phosphate 3-epimerase, ATP synthase beta subunit, thioredoxin-disulfide reductase | Liu et al., 2015 |
| Triticum aestivum L. cv. CS, cv. HX-10 | Roots, Leaves, ISRL | 2 DE, MALDI-TOF/TOF | DRP: Roots 44, leaves 78 and ISRL 34 | 14-3-3, calreticulin-like protein, HSP90, 70, 23.5, RuBisCO LSU. | Hao et al., 2015 |
| Triticum aestivum L. cv. HX10 and NC 47 | Leaves | LTQ-Orbitrap XL MS | TI: HX10 - 173, NC 47-227 DRP: 31 | WCOR615, monosaccharide sensitive protein 2 (MSSP2), wheat aluminum induced 7 (WALI7), Serine/threonine-protein kinase PRP4-like protein, TaABI5, PP2C, H+ -ATPase | Zhang et al., 2014 |
| Triticum aestivum L. cv. Xihan No. 2, cv. Longchun 23 | Leaves | 2-DE, MALDI-TOF/TOF MS | TI: 387 DRP: 148 |
Acid phosphatase, glyceraldehyde-3-phosphate dehydrogenase, peptidyl-prolyl cis-trans isomerase, proteasome subunit alpha, voltage dependent anion channel and S-like RNase | Cheng et al., 2016 |
| Durum wheat cv. Kiziltan, T. dicoccoides lines TR39477, TTD22 | Leaves | 2DE, nanoLC-ESI-MS/MS | TI: 75 DRP: 11 |
TPI, ATP synthase CF1, β-1,3-glucanase, β-1,4-glucanase, XET | Budak et al., 2013 |
| Triticum aestivum L. cv. Yumai 34 | Leaves | 2DE, MALDI-TOF-TOF MS | DRP: 76 | 14−3−3, Ferredoxin-NADP(H) oxidoreductase, SET-domain transcriptional regulator, protein agamous-like 26, glutathione S-transferase 1 | Kang et al., 2012 |
| Triticum aestivum L. cv. Jimai 22 | Grains | 2DE, MALDI-TOF-TOF MS | TI: 136 DRP: 89 |
Cu/Zn SOD, glutathione transferases, myo-inositol-1-phosphate synthase, ATP synthase, HSP70 | Qin et al., 2014 |
| Triticum durum Desf. cv. Waha, Oued Zenati, cv. Djenah Khetifa | Callus | D-2DE, MALDI-TOF-TOF MS | DRP: 16 | Glyceraldehyde-3-phosphate dehydrogenase, Globulin 1S, 3A, peroxidases | Kacem et al., 2016 |
| Triticum turgidum spp. Durum cv. Ciccio, cv. Svevo | Grains | 2 DE, nanoLC-ESI-IT-MS/MS | DRP: 24 | HMW glutenin x-type subunit Bx7, ω-5-gliadin, triticin | Giuliani et al., 2015 |
| Triticum aestivum L. cv. Kauz, cv. Janz | Grains | 2 DE, MALDI-TOF-TOF MS | DRP: 153 | Sucrose synthase, triticin, catalase isozyme 1, WD40 repeat protein, LEA and alpha-amylase inhibitors, GAPDH | Jiang et al., 2012 |
| Triticum aestivum L. cv. Chinese Spring, Ningchun 4 | Grains | 2 DE, MALDI-TOF-TOF MS | TI: 152 Unique proteins 58 | Sucrose synthase, triticin precursor, cytosolic malate dehydrogenase, cytoplasmic aldolase, GAPDH, cytosolic 3-phosphoglycerate kinase | Ge et al., 2012 |
| Triticum aestivum L. cv. Vinjett | Leaves | 2DE, MALDI-TOF MS, MS/MS | TI: 400 | Rubisco LSU, peptidyl-prolyl cis-trans isomerase CYP38, 4-nitrophenyl phosphatase, PSII stability/assembly factor HCF136, Thioredoxin-like protein, CDSP32 | Wang et al., 2014 |
| Triticum aestivum L. cv. Vinjet | Grains | 2DE, MALDI-TOF-TOF MS | TI:250, 100 | LEA, peroxiredoxins and a-amylase/trypsin inhibitors, 3-phosphoglycerate kinase | Yang et al., 2011 |
| Triticum turgidum L. var. durum cv. Borgia, MD-597 | Leaves | 2DE, MALDI-TOF-TOF MS | TI:182 | Oxygen-evolving enhancer protein 2, catalase-1 (CATA1) | Peremarti et al., 2014 |
| Triticum aestivum L. cv. N14, cv. N49 | Stem | 2DE, MALDI TOF/TOF or nano LC-ESI-Q-TOF MS/MS | DRP: 135 | Rubisco LSU, MFP1 attachment factor 1, Tubulin folding cofactor A, thioredoxin H-type (TRX-H), Glyceraldehyde 3-phosphate dehydrogenase | Bazargani et al., 2011 |
| Triticumaestivum L. cv. Chinese Spring, cv. NC47 | Leaves | 2DE, MALDI-TOF/TOF-MS | DRP: 101 (Unique proteins: 77) | Dehydroascorbate reductase (DHAR), ascorbate peroxidase (APX), Cu/Zn superoxide dismutase (SOD), 2-Cys peroxiredoxin and a fibrillin-like protein, chitinase 2, HSP70, RuBisCO LSU | Cheng et al., 2015 |
| RICE | |||||
| Oryza sativa L. cv. IR64 | Roots | nanoLC-MS/MS | TI: 1487 | sHSP II 17.8, Root PR-10, Tim13, CAT-A, Peroxidase 22 | Mirzaei et al., 2012b |
| Oryza sativa L. cv. Nipponbare | Leaves | nanoLC-MS/MS | TI: 1548 | PIPs protein, GTP binding protein, Ras-related protein RIC1, ethylene-responsive small GTP, L-ascorbate peroxidase 2 | Mirzaei et al., 2012a |
| Oryza sativa L. var. Rasi | Apoplastic | 2-DE, LC-MS/TOF | TI: 250 DRP: 192 |
Thioredoxin M, 2-cys peroxiredoxin, Hsp70/DnaK, 14-3-3 protein, oryzacystatin, phosphoribulokinase | Pandey et al., 2010 |
| Oryza sativa L. cv. CT9993, cv. IR62266 | Leaves | 2-DE, MALDI-MS/ESI-Q-TOF-MS/MS | DRP: 42 | actin depolymerizing factor, translational initiation factor EF-Tu, rubisco activase isoforms, chloro-plast fructose-1,6-bisphosphate aldolase, glutathione dehydro ascorbate reductase | Salekdeh et al., 2002 |
| Oryza sativa L. cv. Nipponbare | Leaves | 2-DE | DRP: 18, Phosphoproteins: 10 | ABA, LEA, chloroplast Cu–Zn SOD, Ribosomal protein, NAD-malate dehydrogenase | Ke et al., 2009 |
| Oryza sativa L. IR64, Moroberekan | Anthers | 2-DE, MALDI-TOF-MS/ESI-TOF MS/MS | TI: 93 DRP: 35 |
Peroxiredoxin, Ascorbate peroxidase, Putative actin binding protein, Glutathione S-transferase 2, Adenosine kinase 2 | Liu and Bennett, 2011 |
| Oryza sativa L.cv. Heena | Roots | 2-DE, MALDI-TOF MS-MS | TI: 125 DRP: 78 |
malate dehydrogenase, succinyl-CoA, pyruvate dehydrogenase, cysteine synthase, prolyl endopeptidase | Agrawal et al., 2016 |
| Oryza sativa L. cv. Nipponbare, IAC1131 | Leaves | nanoLC–MS/MS, LTQ-XL ion-trap MS | DRP: 40 (Label free) DRP: 114 (TMT label) |
ClpB1, Hsp17.9, Hsp18.6, HSP 70, RuBiscCO LSU, chaperone protein ClpD1 | Wu et al., 2016 |
| Oryza sativa L. ssp. Indica cv. Zhenshan97B, ssp. Japonica cv. IRAT109 | Leaves | 2-DE, MALDI-TOF MS | DRP: 17 (Zhenshan) DRP: 20 (IRAT109) |
Putative glycine hydroxymethyltransferase, Ribulose bisphosphate carboxylase/oxygenase activase, chloroplast Cu–Zn SOD, Dehydroascorbate reductase, Putative ATP synthase beta subunit | Ji et al., 2012 |
| Oryza sativa L. ssp. Japonica cv. Yongyou 8 | Spikelets | iTRAQ, LC-MS/MS | TI: 1207 DRP: 185 |
Histone H3, Ribulose bisphosphate carboxylase, Thioredoxin H-type, Ras-related protein RIC1 | Dong et al., 2014 |
| Oryza sativa L. var. japonica | Leaves | 2-DE, MALDI-TOF/TOF MS | DRP: 15 | Ribulose-1,5-bisphosphate carboxylase/oxygenase activase, Drought-induced S-like ribonuclease, Putative remorin 1 Protein, Ascorbate peroxidase | Rabello et al., 2014 |
| Oryza sativa L. cv. IRAT109 | Leaves | 2-DE, MALDI-TOF/TOF MS | DRP: 71 | HSP 90, HSP70, Putative chaperonin 60 β, Dehydroascorbate reductase, Putative peptide methionine sulfoxide reductase | Shu et al., 2011 |
| Oryza sativa L. cv. Zhonghua 8, Nipponbare | Seedling | 2-DE, MALDI-TOF MS | DRP: 15 | dnaK-type molecular chaperone, Endosperm luminal binding protein (BiP), Uroporphyrinogen decarboxylase | Zang and Komatsu, 2007 |
| Oryza sativa L. cv. IR64 | Leaves | nanoLC−MS/MS LTQ-XL | TI: 1383 | Cyt b6-f complex Fe-S subunit, Ferritin, Succinate dehydrogenase, Putative hexose transporter, proteasome subunit beta type, drought induced S-like ribonuclease, HSP 101, succinate dehydrogenase, ATP-dependent Clp protease | Mirzaei et al., 2014 |
| Oryza sativa L. cv. IR64 | Peduncles | 2-DE, MALDI-TOF MS | DRP: 31 | Putative actin-binding protein, LEA type-1 protein, GSH-dependent dehydroascorbate Reductase, S-adenosylmethionine synthetase | Muthurajan et al., 2011 |
| Oryza sativa L. var. japonica, cv. Prata Ligeiro, IRAT20 | Root | 2-DE, MALDI-TOF/TOF MS | TI: 463 (Prata Ligeiro) TI: 522 (IRAT20) | GSH-dependent dehydroascorbate reductase 1, Putative superoxide dismutase [Cu-Zn], Triosephosphate isomerase, Ascorbate peroxidase | Rabello et al., 2008 |
| Oryza sativa L. cv. Zhonghua 8, Nipponbare | Leaf sheath | 1D-IEF, 2DE-PAGE, CBB stained gels analysed in ImageMaster 2D Elite software | TI: 698 | Serine hydroxylmethyltransferase I, Superoxide dismutase, Purative actin depolymerizing factor, Chloroplast ATPase, actin depolymerizing factor | Ali and Komatsu, 2006 |
| MAIZE | |||||
| Zea mays L. (Lc and Io) | Leaves | 2-DE, Kepler package | TI: 413 DRP: 78 |
RAB17 responsive to ABA, enolase, beta-glucosidase, putative cytoplasmic NAD-malate dehydrogenase, phosphoribulokinase, chloroplastic Fru 1,6-bisphosphate aldolase | Riccardi et al., 1998 |
| Zea mays L. (Io and F2) | Leaves | IEF, 2-DE scanned, transmittance values into optical density values and relative intensities calculated | DRP: 46 | ABA45, oxygen evolving enhancer protein 1 (OEE1), OSR40, malate dehydrogenase, a cystein synthase, phosphoribulokinase | Riccardi et al., 2004 |
| Zea mays L. cv. Nongda 108 | Embryo | 2-DE, MALDI-TOF-TOF/MS | DRP: 111 | Malate dehydrogenase, Ascorbate peroxidase, Carbomoylphosphate synthase, Superoxide dismutase, Triosephosphate isomerase | Huang et al., 2012 |
| Zea mays L. wild type: Vp5 | Leaves | iTRAQ, LC-ESI MS/MS | TI: 7051 DRP: 150 |
Ribose-phosphate Pyrophosphokinase 4, Photosystem I reaction center subunit XI, Photosystem II reaction center protein L, 4F5 protein family protein, Peroxidase | Zhao et al., 2016a |
| Zea mays L. cv. Zhengdan 958 | Leaves | iTRAQ, LC-ESI MS/MS | DRP: 65 | RAB17 protein/A3KLI0, Dehydrin/C4J477, ABA-responsiveprotein/K7TFB6, Photosystem I reaction center subunit V/B4G1K9, Asparagine synthetase, Stachyose synthase | Zhao et al., 2016b |
| Zea mays L. wild type: Vp5 | Leaves | iTRAQ, LC-ESI MS/MS | DRP: 379 (phosphopeptides) | Phosphoenolpyruvate carboxylase, Na+/H+ antiporter, Ubiquitin ligase protein cop1, arginine serine rich splicing factor rsp41, heterogeneous nuclear ribonucleoprotein r-like | Hu et al., 2015a |
| Zea mays L. cv. Zhengdan 958 | Leaves | iTRAQ, LC-MS/MS | DRP: 149 (phosphopeptides) | Serine threonine-protein kinase wnk4-like/K7TZQ1, Phospholipase c/B4FY17, Phosphoenolpyruvate carboxylase/E9NQE1, Probable-trehalose-phosphate synthase/K7V0H0, RAB17protein/A3KLI0, Phosphoenolpyruvate carboxykinase | Hu et al., 2015b |
| Zea mays L. Inbred line B104 | Leaves | nanoLC−MS/MS | TI: 536 DRP: 18 (phosphoproteins) |
Histidine decarboxylase, adenosyl homocysteinase, Exocyst complex component SEC5A, RANBP2-like and GRIP domain-containing protein | Vu et al., 2016 |
| Zea mays L. cv. Tolerant CE704, cv. Sensitive 2023 | Leaves | 2DE-iTRAQ, LC-MALDI TOF/TOF | DRP: 129 (Tolerant) DRP: 97 (Sensitive) |
Dehydrin RAB-17, HSP26, Ribonucleoprotein A, HSP17.4 | Benešová et al., 2012 |
| Zea mays L. cv. FR697 | Xylem sap | 2DE, LC-MS/MS | DRP: 39 | Xyloglucan endotransg-lycosylase homolog, Peroxidase 52 precursor, Endonuclease, Secretory protein | Alvarez et al., 2008 |
| Zea mays L. Inbred tolerant lines 81565 and 200B | Leaves | 2DE-IEF-PAGE- MALDI-TOF-MS | TI: 500 DRP: 58 |
cinnamyl alcohol dehydrogenase, caffeate O-methyltransferase, Cytochrome protein 96A8 | Hu et al., 2009 |
| Zea mays L. (Io and F2) | Leaves | 2DE, LC-MS/MS | TI: 300 | methylenetetrahydrofolate reductase, S-adenosyl-L-Met (SAM) synthases, 5-methyltetrahydrafolate | Vincent et al., 2005 |
| Zea mays L. cv. Sensitive B73, cv. Tolerant Lo964 | Maize Kernels | iTRAQ, LC-MS/MS | DRP: 78 | Dehydrin, RAB-17, Late embryogenesis abundant protein LEA 14-A, Stress-inducible membrane pore protein, Protein kinase C inhibitor, Thioredoxin H-type, Superoxide dismutase-4A, ABA-responsive protein, | Yang et al., 2014 |
| BARLEY | |||||
| Hordeum vulgare L. cv. Arta, cv. Keel | Leaves | 2-DE, MALDI-TOF/TOF-MS | NA | No significant identification was reported | Rollins et al., 2013 |
| Hordeum vulgare L. acc. no. 15141, 15163 | Leaves | 2-DE, MALDI-TOF-MS | DRP: 22 DRP: 27 |
Methionine synthase, ATP synthase subunit alpha, glyoxysomal malate dehydrogenase, heat shock protein 90, ATP-dependent Clp protease | Ashoub et al., 2013 |
| Hordeum vulgare L. ssp. Spontaneum cv. XZ5, cv. XZ54, cv. ZAU3 | Leaves | 2-DE, MALDI-TOF/TOF-MS | DRP: 38 | Ribulosebisphosphate carboxylase small chain clone 512, Glutathione S-transferase 1, ATP synthase beta subunit, Transketolase, chloroplast | Wang et al., 2015 |
| Hordeum vulgare L. Jau-83, Frontier-87, Jau-87, Haider-93, Sanober-96 and Soorab-96 | Shoots | 2-DE, MALDI-TOF-MS, nanospray LTQ XL Orbitrap MS | Sensitive DRP: 31, Tolerant line DRP: 28 | ATPase subunit E, photosystem I reaction center II, Ptr ToxA-binding protein, ATP synthase CF1 alpha subunit, glutathione transferase, oxygen evolving complex precursor, HSP70 | Kausar et al., 2013 |
| Hordeum vulgare L. cv. Amulet | Leaves and crowns | 2DE, MALDI-TOF/TOF | DRP: 105 | Annexin, Flavoprotein wrbA-like isoform 1, Putative r40c1 protein (ricin B lectin domain), Glutathione peroxidase, Glutathione S-transferase 6, chloroplastic; Methionine synthase, UDP-glucose 6-dehydrogenase | Vítámvás et al., 2015 |
| Hordeum vulgare L. cv. Maresi, cv. Cam/B1//CI08887/CI05761 | Leaves and roots | 2DE, MALDI-TOF and MALDI-TOF/TOF | DRP: 121 leaves, 182 roots | sHSP (17.6 kDa,16.9 kDa) cold-regulated protein (COR),Mitochondrial pyruvate dehydrogenase E1 subunit alpha (PDHA1), NADP dependent malic enzyme (NADP-ME), hydroxy acid oxidase (HAO, also known as glycolate oxidase), oxalate oxidase (OXO), ascorbate peroxidase (APX) | Chmielewska et al., 2016 |
| Hordeum vulgare L. cv. Golden Promise, cv. Basrah | Leaves and roots | 2DE, MALDI-TOF | DRP: 24 leaves, 45 roots | 14-3-3, GTP-binding protein Rab2, cytochrome p450, methionine synthase and S-adenosylmethionine synthase | Wendelboe-Nelson and Morris, 2012 |
| SORGHUM | |||||
| Sorghum bicolor L. Moench cv. 11434, cv.11431 | Leaves | 2-DE DIGE, MALDI-TOF-MS | DRP: 18 DRP: 23 |
Methionine synthase, S-adenosylmethionine Synthase, P-(S)- hydroxymandelonitrile lyase, PEPC, PPDK, Hsp60 | Jedmowski et al., 2014 |
| PEARL MILLET | |||||
| Pennisetum glaucum. (L.) R. Br. cv. ICTP 8203 | Leaves | nanoESI-LC-MS/MS | TI: 1208 DRP: 120 |
Thioredoxin-dependent peroxidase 2, Glyceraldehyde 3-phosphate dehydrogenase A subunit 2, ribosomal proteins, prohibitin 3, chlorophyll a/b-binding protein | Ghatak et al., 2016 |
| Roots | TI: 1095 DRP: 25 |
Cytochrome C1 family, UDP-glucosyl transferase, Annexins, Lipoxygenase, Phosphoinositide-specific phospholipase C (PI-PLC), NAD(P)-linked oxidoreductase superfamily protein | Ghatak et al., 2016 | ||
| Seeds | TI: 1299 DRP: 10 |
LEA, heat shock proteins 70, 21 kDa, threonine synthase | Ghatak et al., 2016 | ||