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. 2017 Jun 2;8:113. doi: 10.3389/fendo.2017.00113

Figure 1.

Figure 1

GnRH induces bleb formation in LβT2 cells and GnRH receptor (GnRHR) is present in the blebs. (A) Images from a confocal microscopy time-lapse movie of serum-starved LβT2 gonadotrope cells treated with GnRH (10 nM) (0–30 min). The treatment resulted in bleb formation. The scale bar is 10 µm. (B) GnRHR is present in the blebs. Serum-starved LβT2 cells were subjected to time laps confocal microscopy. Addition of GnRH (10 nM) (0–10 min) to LβT2 cells transfected with GnRHR-mCherry resulted in bleb formation, while GnRHR is present in the blebs. The scale bar is 5 µm. (C) GnRHR mediates the formation of the blebs by GnRH. Serum-starved LβT2 cells were incubated with GnRH (10 nM) (30 min), or preincubated first with 100 nM GnRH antagonist (cetrorelix acetate) for 30 min followed by GnRH (10 nM) (GnRH antagonist + GnRH) for additional 30 min. Cells were imaged using confocal microscope and the scale bar is 10 µm. (D) Quantitation of blebbing cells in control, GnRH and GnRH antagonist + GnRH. Images of at least 10 fields were taken for each treatment, and the bars are mean ± SEM from 3 experiments.