Table 3.
Binding affinities of prosurvival Bcl-2 proteins to BH3 domain peptides of proapoptotic Bcl-2 proteins
NB, no binding; n/a, not applicable.
| Poxviral Bcl-2 |
Asfarviridae Bcl-2 A179Lb | Herpesviral Bcl-2 |
Cellular Bcl-2 |
|||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| SPPV14a | M11La | MVAF1La | DPV022b | FPV039b | BHRF1b | KsBcl-2c | M11b | Bcl-2a | Bcl-wa | Bcl-xLa | Mcl-1a | A1b | ||
| Bad | >2,000 | >1,000 | NB | NB | 653 | 258 | >2,000 | >1,000 | n/a | 16 | 30 | 5.3 | >100,000 | 15,000 |
| Bid | 341 | 100 | NB | NB | 2 | 26 | 109 | 112 | 232 | 6,800 | 40 | 82 | 2,100 | 1 |
| Bik | >2,000 | >1,000 | NB | NB | 30 | 190 | >2,000 | >1,000 | n/a | 850 | 12 | 43 | 1,700 | 58 |
| Bim | 26 | 5 | 250 | 340 | 10 | 6 | 18 | 29 | 131 | 2.6 | 4.3 | 4.6 | 2.4 | 1 |
| Bmf | 67 | 100 | NB | NB | 16 | 254 | >2,000 | >1,000 | 300 | 3 | 9.8 | 9.7 | 1,100 | 180 |
| Hrk | 63 | >1,000 | NB | NB | 24 | 1,487 | >1,000 | >1,000 | 719 | 320 | 49 | 3.7 | 370 | 46 |
| Noxa | >2,000 | >1,000 | NB | NB | 28 | 1,575 | >2,000 | >1,000 | 132 | >100,000 | >100,000 | >100,000 | 24 | 20 |
| Puma | 65 | >1,000 | NB | NB | 24 | 31 | 70 | 69 | 370 | 3.3 | 5.1 | 6.3 | 5 | 1 |
| Bak | 46 | 50 | 4,300 | 6,930 | 76 | 29 | 150 | <50 | 76.3 | >1,000 | 500 | 50 | 10 | 3 |
| Bax | 32 | 75 | 1,850 | 4,040 | 76 | 26 | 1,400 | 980 | 690 | 100 | 58 | 130 | 12 | n/a |
a Affinity measured in nm by surface plasmon resonance (KD).
b Affinity measured in nm by isothermal calorimetry (KD).
c Affinity measured in nm by fluorescence polarization (IC50).