Figure 10.

Comparison of FlAsH measurements with two other measurements of C2-α-syn fibril formation following seeded fibril growth for 96 h further validates SeGMA assays. Homologous and heterologous mixtures of 11.5 μg/ml seeds and 500 μg/ml monomer were incubated at 37 °C without agitation for 96 h. Fibril formation for each of the homologous and heterologous seed-monomer combinations was assessed by FlAsH fluorescence (A, D, and G); BCA sedimentation assay, which measures fibril concentration based on the change in the supernatant concentration of C2-α-syn after centrifugation at 100,000 × g (B, E, and H); and ThioT fluorescence (C, F, and I) (mean ± S.D. (error bars), n = 3, independent experiments = 2).